Examination of smears for tubercle bacilli by Fluorescence Microscopy

IN underdeveloped countries, laboratory facilities for the bacteriological diagnosis of tuberculosis are at present, very limited. Cultural methods are unlikely to be used on a large scale for many years to come. It is, therefore, important to investigate the most economical method of examining smears for tubercle bacilli. Fluorescence microscopy was introduced by Hagemann (1937) and has since been described by many authors, including Tanner (1941, 1948), Lind and Shaughnessy (1941), Lempert (1944), Norman and Jelks (1945), Clegg and Foster-Carter (1946), Wilson (1952), Von Haebler and Murray (1954), and Needham (1957). The great advantage claimed for this method is that stained bacilli can be detected using a much lower magnification than with the usual Ziehl-Neelsen method. Considerable time is saved in examining smears and larger areas can be searched. The method has not been widely employed for two reasons. In the first place, the light source must be very bright and many of the optical systems described previously have only supplied sufficient light if the equipment was used in a darkened room. Secondly, some workers (Ritterhoff and Bowman, 1945; Kuster, 1939; Holm and Plum, 1943) consider that false positive results can be obtained, since some smears may contain small naturally fluorescent particles which can be confused with bacilli. Equipment for fluorescence microscopy that can be used in normal daylight has been in use at the Tuberculosis Chemotherapy Centre, Madras, for over two years. When it was first introduced, a comparison between this method and the conventional Ziehl-Neelsen method was undertaken to test their relative sensitivities, and to see whether fluorescence microscopy yielded false positive results. The results of this comparison are described

Pengaruh Penggunaan Media Google Earth dan Peta terhadap Peningkatan Hasil Belajar Geografi

The aims of his research were: (1) to describe the influence in the use of google earth media to increase the result of students' learning outcomes of geography for the material of Southeast Asia at SMA 14 Bandar Lampung. (2) to find out the difference of students' learning outocomes for the material of Southeast Asia nation using google earth learning media by using map at XI IPS class of SMAN 14 Bandar Lampung. The population of this research is the students of XI IPS Negeri 14 Bandar Lampung who were 332 students. The sample of the research was carried out by cluster sampling techniques so the researcher got XI IPS1 class (experimental class) and XI IPS II Class (control class) as samples. The researcher used Independent T-test as the technique of data analysis for the results of students' learning outcomes. The result of the research indicated that for the first hypothesis, the significance value was 0.183 so it can be inferred that significance value was >0.05, then Ho was accepted. For the second hypothesis, it was 0.001 < 0.05, then Ho was denied so it can be concluded that there is a difference of students' learning outcomes whose learning used google earth media the first meeting at SMAN 14 Bandar Lampung which means the stusents who have improvement in the second meeting and student learning outcomes in the used os google earth media are higher that in the use of map both at the first and second meeting

The role of intention and self-efficacy on the association between breastfeeding of first and second child, a Danish cohort study

Abstract Background The impact of parity on breastfeeding duration may be explained by physiological as well as psychosocial factors. The aim in the present study was to investigate the mediating influence of intention and self-efficacy on the association between the breastfeeding duration of the first and the following child. Methods A 5-year Danish cohort study with data from online questionnaires was used. Data came from 1162 women, who participated in the “Ready for child” trial in 2006–7 and gave birth to their second child within 5 years in 2011–3. Analysis included multiple regression models with exclusive/any breastfeeding duration of first child as the exposure variables, intention and self-efficacy measured as mediators, and exclusive/any breastfeeding duration of the second child as the outcome variables. Results Duration of exclusive breastfeeding of the first child was significantly associated with exclusive breastfeeding duration of the second child (p <  0.001) and with the self-reported intention and self-efficacy in the ability to breastfeed the second child (p <  0.001). The exclusive breastfeeding period was slightly longer for the second child. Self-efficacy and intention mediated the association between breastfeeding duration in the first and second child. Together the two factors explained 48% of the association in exclusive breastfeeding and 27% of the association in any breastfeeding between the first and second child. Conclusion Due to a reinforcing effect of intention and self-efficacy, breastfeeding support should focus on helping the first time mothers to succeed as well as to identify the second time mother with low self-efficacy and additional need for support

Summer Sea Ice Concentration, Motion, and Thickness Near Areas of Proposed Offshore Oil and Gas Development in the Canadian Beaufort Sea – 2009

This study was motivated by the potential development of offshore oil exploration leases in the Canadian Southern Beaufort Sea, an area within the Inuvialuit Settlement Region. Sea ice concentration, extent, motion, and thickness data are vital to the success of potential oil operations in this region, and relevant data cannot be gleaned from larger-scale hemispheric studies. We therefore undertook regionally specific sea ice analyses in the southern Beaufort Sea during the summer drilling season (July, August, and September) in 2009 and over the long-term (1996 – 2010). On average, the Canadian oil lease areas contain mostly old sea ice during the drilling season and have not experienced significant decreasing trends in total or old sea ice. The average sea ice motion in the region for the period was anti-cyclonic at 20 – 25 cm·s-1, acting to transport sea ice southward toward the lease areas. Summer 2009 was used as a case study of regional ice concentration, motion, and thickness and to compare September sea ice thickness measurements to data collected in April 2009. In the summer of 2009, old sea ice was the predominant ice type in the lease areas. Sea ice motion was anti-cyclonic and faster than the long-term average, reaching 60 cm·s-1 west of Banks Island and across the north end of the lease areas. September 2009 sea ice thickness (mean = 1.03 m, σ = 0.97 m) was modal about the 0.20 – 0.29 m thickness bin. The sea ice thickness distri­bution was spatially variable, with the thickest ice occurring at the north end of the study area, in an area dominated by high old ice concentrations. Ice thicknesses greater than 10 m (the upper limit our instruments could measure) were encountered. Thinner sea ice predominated at the periphery of the core Beaufort Sea multi-year pack. Near the oil lease areas, the sea ice thickness distributions were shifted left on the histogram in comparison to those farther north, resulting in a greater proportion of relatively thick sea ice due to the thermodynamic loss of thinner (&lt; 1.5 m) first-year ice during its southward movement. After enduring a summer’s melt, however, this thicker ice at the south end of the study region had thinned in comparison to the ice at the north end.La présente étude a été motivée par la mise en valeur potentielle des concessions d’exploration pétrolière au large de la mer de Beaufort, dans la partie sud canadienne, un endroit qui fait partie de la région désignée des Inuvialuit. Les données relatives à la concentration, à l’étendue, au déplacement et à l’épaisseur de la glace de mer sont essentielles à la réussite de l’exploitation éventuelle du pétrole dans cette région, et les données pertinentes ne peuvent être dépouillées à partir d’études hémisphériques réalisées à grande échelle. Par conséquent, nous avons entrepris de faire des analyses particulièrement régionales de la glace de mer du sud de la mer de Beaufort pendant la saison de forage d’été (juillet, août et septembre) en 2009 de même que sur une plus longue période (1996-2010). En moyenne, les régions visées par les concessions pétrolières canadiennes renferment principalement de la vieille glace de mer pendant la saison de forage, et elles n’enregistrent pas d’importantes tendances à la baisse sur le plan de l’ensemble de la glace de mer ou de la vieille glace de mer. Dans la région, le déplacement moyen de la glace de mer pendant la période était anticyclonique à 20 25 cm·s-1, ce qui avait pour effet de transporter la glace de mer vers le sud et vers les concessions. L’été 2009 nous a servi d’étude de cas en matière de concentration, de déplacement et d’épaisseur de la glace régionale, et nous a permis de comparer les mesures de l’épaisseur de la glace de mer de septembre aux données recueillies en avril 2009. À l’été 2009, la vieille glace de mer représentait le type de glace prédominant dans les concessions. Le déplacement de la glace de mer était anticyclonique et se faisait plus vite que la moyenne à long terme, atteignant ainsi 60 cm·s-1 à l’ouest de l’île Banks et à la hauteur du nord de la zone de concessions. En septembre 2009, l’épaisseur de la glace de mer (moyenne = 1,03 m, σ = 0,97 m) était modale à la hauteur de la classe de l’épaisseur 0,20 – 0,29 m. La répartition de l’épaisseur de la glace de mer variait en fonction de l’emplacement, la glace la plus épaisse se trouvant du côté nord de la région étudiée, dans une zone dominée par de fortes concentrations de vieille glace. La glace atteignait des épaisseurs de plus de 10 m (la limite maximale que nos instruments pouvaient mesurer) par endroits. Une glace de mer plus mince prédominait la périphérie du noyau de la banquise pluriannuelle de la mer de Beaufort. Près de la zone de concessions pétrolières, les répartitions d’épaisseurs de glace de mer se sont déplacées vers la gauche sur l’histogramme comparativement à celles plus au nord, ce qui a donné une plus grande proportion de glace de mer relativement épaisse en raison de la perte thermodynamique de la glace plus mince de première année (&lt; 1,5 m) pendant son déplacement vers le sud. Cependant, après avoir enduré la fonte d’un été, la glace plus épaisse du côté sud de la région à l’étude s’était amincie compara­tivement à la glace se trouvant du côté nord

Whole-blood transcriptomic signatures induced during immunization by chloroquine prophylaxis and Plasmodium falciparum sporozoites

A highly effective vaccine that confers sterile protection to malaria is urgently needed. Immunization under chemoprophylaxis with sporozoites (CPS) consistently confers high levels of protection in the Controlled Human Malaria infection (CHMI) model. To provide a broad, unbiased assessment of the composition and kinetics of direct ex vivo human immune responses to CPS, we profiled whole-blood transcriptomes by RNA-seq before and during CPS immunization and following CHMI challenge. Differential expression of genes enriched in modules related to T cells, NK cells, protein synthesis, and mitochondrial processes were detected in fully protected individuals four weeks after the first immunization. Non-protected individuals demonstrated transcriptomic changes after the third immunization and the day of treatment, with upregulation of interferon and innate inflammatory genes and downregulation of B-cell signatures. Protected individuals demonstrated more significant interactions between blood transcription modules compared to non-protected individuals several weeks after the second and third immunizations. These data provide insight into the molecular and cellular basis of CPS-induced immune protection from P. falciparum infection

Shielding of a moving test charge in a quantum plasma

The linearized potential of a moving test charge in a one-component fully degenerate fermion plasma is studied using the Lindhard dielectric function. The motion is found to greatly enhance the Friedel oscillations behind the charge, especially for velocities larger than a half of the Fermi velocity, in which case the asymptotic behavior of their amplitude changes from 1/r^3 to 1/r^2.5. In the absence of the quantum recoil (tunneling) the potential reduces to a form similar to that in a classical Maxwellian plasma, with a difference being that the plasma oscillations behind the charge at velocities larger than the Fermi velocity are not Landau-damped.Comment: 9 pages, 11 figures. v3: Fixed typo, updated abstrac